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Application of the white-rot fungus Trametes sp. (C3) laccase in the removal of acetaminophen from aqueous solutions

Abstract

Effluents containing drugs originating from hospitals, veterinary clinics, residences, and pharmaceutical industries, pass through the sewage treatment system and are discharged into the aquatic environment, causing water contamination. The methods used in sewage treatment are ineffective for these types of micropollutants. Laccase, an enzyme produced by white-rot fungi, has been used to successfully remove drugs in waters. The objective of this work was to evaluate the removal of acetaminophen from aqueous solutions by the fungus Trametes sp. (C3). Ethanol showed efficiency in inducing the production of the laccase enzyme and in vitro removal tests the amount of drug decreased by 76 %. The use of the 1-hydroxybenzotriazole (HBT) as mediator caused a lower removal rate than the assay without its presence, indicating that it acted as an inhibitor of degradation. In in vivo tests, there was a 79 % removal rate of acetaminophen on the fourth day of culture. Toxicity assays showed that after the action of the laccase there was toxicity to the larval stage of Artemia salina.

article Article

date_range 2024

language English

link Link of the paper

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Sybuia Priscila

Contato Alex

De araujo Caroline

Zanzarin Daniele

Maciel Giselle

Pilau Eduardo

Peralta Rosane

De souza Cristina

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Featured Keywords

Acetaminophen

Removal

Laccase

Trametes

Emerging pollutants

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Effect of corrosion on flexural strength of reinforced concrete beams with polypropylene fibers

Abstract

An experimental study was performed to investigate the effects of polypropylene fibers on uncorroded and corroded reinforced concrete beams. Three different volume fractions of polypropylene fibers having 0, 0.5, and 1.5%, were tested at four corrosion levels of 0% and approximately 5, 7, and 9%. A full scale of an accelerated corrosion pool was used for the accelerated corrosion process. Reinforced concrete beams were used for an under monotonic bending test. The contribution of the actual corrosion levels of transverse and longitudinal reinforcement bars to the total corrosion levels were obtained from reinforcement bars fully extracted from concrete. Flexural strength, bond-slip, and moment-curvature relationships were examined for uncorroded and corroded reinforced concrete beams. A new model was developed to predict the flexural strength of corroded reinforced concrete beams. The proposed model for predicting the residual flexural strength of corroded beams was compared with test data published in previous studies. Furthermore, a novel model is presented for improved predictions between the actual and theoretically estimated corrosion mass losses, based on Faraday’s law, with the aid of fully extracted reinforcement bars. The model used to predict the flexural strength of corroded reinforced concrete beams with large sizes demonstrated good agreement with current and previously published literature data. In the case of corroded beams comprising differing amounts of polypropylene fibers, the performance of the corroded beams was limited by a fiber volume fraction of 1.5% at low corrosion levels.

article Article

date_range 2018

language English

link Link of the paper

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