New Insights into Secondary Metabolite Potential in <i>Hypericum Perforatum</i> , UPLC‐ESI‐MS/MS to Novel Phytochemical Discovery with Bioactivity Assessment

Abstract Different anatomical components of Hypericum perforatum ( H. perforatum ) have been utilized by humans for generations as a natural remedy with pharmacological attributes. This work aimed to investigate the secondary metabolite potential of H. perforatum from algeria using LC‐ESI‐MS/MS to determine its phytochemical profile. LC‐ESI‐MS/MS analysis revealed 22 components, with isoquercitrin being the most abundant bioactive compound at a concentration of 4162 µg/g. The total phenolic and flavonoid contents were also quantified, with TPC equal 171.54 ± 0.79 mg GAE/g and TFC equal 144.26 ± 14.3 mg QE/g. The antioxidant capacity of the extract was evaluated using in vitro assays, showing strong activity with an ABTS IC 50 of 0.173 mg/mL. The antiproliferative potential of the methanolic extract was assessed by the MTT assay on CAPAN‐1, DLD‐1, and the healthy L929 cell line. The extract exhibited significant antiproliferative effects on CAPAN‐1 and DLD‐1 cells, with IC 50 values of 0.807 ± 0.06 mg/mL and 0.953 ± 0.03 mg/mL, respectively, whereas no cytotoxicity was observed on L929 cells. Furthermore, SwissADME was used to evaluate the pharmacokinetics and drug‐likeness of the main compounds. These findings enhance the understanding of H. perforatum and may support its prospective applications in pharmaceutical and cosmetic industries.