Enhancing Biohybrid CO₂ to Multicarbon Reduction via Adapted Whole-Cell Catalysts

Catalytic CO₂ conversion to renewable fuel is of utmost importance to establish a carbon-neutral society. Bioelectrochemical CO₂ reduction, in which a solid cathode interfaces with CO₂-reducing bacteria, represents a promising approach for renewable and sustainable fuel production. The rational design of biocatalysts in the biohybrid system is imperative to effectively reduce CO₂ into valuable chemicals. Here, we introduce methanol adapted Sporomusa ovata (S. ovata) to enhance the slow metabolic activity of wild-type microorganisms to our semiconductive silicon nanowires (Si NWs) array for efficient CO₂ reduction. The adapted whole-cell catalysts enable an enhancement of CO₂ fixation with a superior faradaic efficiency on the poised Si NWs cathode. The synergy of the high-surface-area cathode and the adapted strain achieves a CO₂-reducing current density of 0.88 ± 0.11 mA/cm², which is 2.4-fold higher than the wild-type strain. This new generation of biohybrids using adapted S. ovata also decreases the charge transfer resistance at the cathodic interface and facilitates the faster charge transfer from the solid electrode to bacteria.